- Either type or paste the input protein sequence(s) in FASTA format or upload FASTA files using the Browse button. The server requires one wild-type sequence and one or more mutant protein sequences for analysis.
Note1 : The input sequences should contain only standard amino acids (A, R, N, D, C, Q, E, G, H, I, L, K, M, F, P, S, T, W, Y, V). The wild-type and mutant sequences must have identical sequence lengths.
Note2 : The submitted sequences or the fasta file should not contain any empty lines.The submitted FASTA files should not contain empty lines. At least one amino acid mutation must exist between the wild-type and mutant sequences.
- Click on the Submit button to start the analysis. The webserver uses transformer-based protein language models, intrinsic disorder prediction, and explainable machine learning to investigate mutation-induced perturbations and functional rewiring.
- Upon submission, the prediction results are displayed on the screen, including perturbation profiles, disorder redistribution, mutation impact scores, functional hotspots, saturation mutagenesis results, and candidate druggable residues. Users may optionally download the generated output files and plots.
- The server additionally performs saturation mutagenesis-based novel mutation prediction to identify highly disruptive mutations and highly interactive functional residues potentially associated with allosteric regulation and therapeutic targeting.
- The Test Run button allows users to analyze an example dataset and explore the corresponding mutation perturbation and hotspot analysis results generated by Mutagenix.